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首页 > 蛋白生物学 > 蛋白Marker > 变性蛋白Marker

三色预染高分子量蛋白Marker  IV(20-950kDa)

三色预染高分子量蛋白Marker IV(20-950kDa)

产品编号:RTD6171

产品规格:10次

数量
价格 ¥400

三色预染高分子量蛋白质Marker IV(20-950 kDa)

TriColor Prestained High Molecular Weight Protein Marker IV(20-950 kDa) Ver.761070-2.0

货号

名称

规格

RTD6171

三色预染高分子量蛋白质Marker IV(20-950 kDa)

10次(100 μl)

● 贮存、运输及效期:

-20°C贮存,湿冰运输。按规定条件保存,有效期为12个月。避免反复冻融。

● 产品简介:

   本产品由8种经过纯化和染料标记的蛋白质组成,表观分子量分别约为950、450、250、150、100、75、45和20 kDa。其中,75 kDa条带为红色,20 kDa条带为绿色,其余条带均为蓝色。

本产品无需加热或额外处理,可直接用于SDS-PAGE电泳,适用于监测蛋白质电泳过程、估算目的蛋白的分子量以及观察Western Blot转膜效果。本产品适用于多种SDS-PAGE凝胶体系,不同凝胶体系中的条带迁移位置及分离效果可能存在差异。每支100 μl,按每次上样10 μl计算,可使用约10次。

技术规格

条带数量

8条

浓度

0.2-0.4 μg/μl

上样前处理

溶化混匀后使用,不要加热

推荐凝胶体系

3–8% Tris-醋酸体系(适用于高分子量蛋白分离,20和45 kDa条带可能集中于电泳前沿附近);

4–12% Bis-Tris体系(适用于宽分子量范围蛋白分离);

4–15% Tris-甘氨酸体系(950 kDa条带可能无法进入分离胶或无法清晰分辨)

推荐上样体积

~10 μl(1mm厚度10齿梳子)

推荐染色方法

无需染色即可观察条带,也可在电泳结束后蛋白染色


● 使用说明:

1. 使用前将产品置于常温溶化,充分混匀后短暂离心,使溶液集中于管底。

2. 本产品已含有上样缓冲液,无需额外添加上样缓冲液或还原剂,也无需加热处理,可直接上样。

3. 根据凝胶厚度及加样孔宽度调整上样量。对于1 mm厚、10齿梳子的凝胶,推荐每孔上样约10 μl,可根据实际需要适当调整。

4. 电泳过程中可直接观察蛋白条带的迁移情况。电泳结束后,无需染色即可观察Marker条带,也可用于Western Blot转膜效果的评估。

5. 本产品各条带的标称分子量为经电泳校准的表观分子量。由于预染染料及不同电泳缓冲体系可能影响蛋白质的迁移率,各条带的实际迁移位置可能存在差异。因此,本产品适用于目的蛋白分子量的初步估算,不建议用于蛋白质分子量的精确定量测定。

实验示例:

3-8%Tris醋酸胶电泳检测结果
凝胶:3-8% RealPAGE Tris-醋酸预制胶(RTD6154-0308P)
1×Tris-醋酸-SDS电泳缓冲液(TA050),内槽加抗氧化剂(TA1510)
恒压120 V 电流变化37-27 mA 93 min
G2085:Servicebio Prestained Protein Marker VI (55-320 kDa)
RTD6151:三色预染高分子量蛋白质Marker III(25-400 kDa)
RTD6171:三色预染高分子量蛋白Marker IV(20-950kDa)
LC5688:Thermo HiMark Unstained Protein Standard(40-500 kDa)
1610363:Bio-Rad Precision Plus Protein Standards Unstained(10-250 kDa)

使用蛋白Marker产品发表部分文章列表:

1. [2021 IF=5.5] Garviecin LG34, a novel bacteriocin produced by Lactococcus garvieaeisolated from traditional Chinese fermented cucumber.

Author: Yurong Gao, Dapeng Li, Shan Liu, Liyuan Zhang

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control 50 (2015) 896e900

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link:http://dx.doi.org/10.1016/j.foodcont.2014.10.040

 

2. [2021 IF=5.5] Purification and partial characterization of M1-UVs300, a novel bacteriocin produced by   isolated from fermented sausage Lactobacillus plantarum.

Author: Yu An, Ying Wang, Xiaoyue Liang, Huaxi Yi, Zhaohang Zuo, Xiaoxi Xu, Dongjie

Zhang, Changqing Yu, Xue Han

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Food Control  Volume 81, November 2017, Pages 211-217

Institution:Key Laboratory of Veterinary Medicine of Heilongjiang Bayi Agricultural University

Paper link:https://doi.org/10.1016/j.foodcont.2017.05.030

 

3. [2021 IF=5.75] The Verticillium dahliae SnodProt1-Like Protein VdCP1 Contributes to Virulence and Triggers the Plant Immune System.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Jingjing Yuan and Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker(1.2-45 kD)

Journal: Frontiers in Plant Science.  Volume 81, November 2017, Pages 211-217

Institution:Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link:https://doi.org/10.1093/jxb/ery351

 

4. [2021 IF=2.99] Purifcation, characterization, and action mechanism of plantaricin DL3, a novel bacteriocin against Pseudomonas aeruginosa produced by Lactobacillus plantarum DL3 from Chinese Suan Tsai.

Author: Xinran Lv,  Yang Lin ,Yu Jie, Mengtong Sun, Bolin Zhang,Fengling Bai,Hongfei Zhao,Jianrong Li

Marker: RTD6127,超低分子量蛋白Marker IV(3.3-45 kD)

Journal: European Food Research and Technology. Vol 244, pages 323–331 (2018)

Institution:College of Biological Science and Biotechnology, Beijing Forestry University

Paper link:https://link.springer.com/article/10.1007/s00217-017-2958-3

 

5. [2021 IF=6.99] Verticillium dahliae Alt a 1-like protein PevD1 targets cotton PR5-like protein and contributes to fungal infection.

Author: Yi Zhang, Yuhan Gao, Yingbo Liang, Yijie Dong, Xiufen Yang, Dewen Qiu

Marker: RTD6110,彩虹超低分子量蛋白Marker (1.2-45 kD)

Journal: Journal of Experimental Botany, Vol 70, Issue 2, 15 January 2019,613-626

Institution:Institute of Plant Protection, Chinese Academy of Agricultural Sciences

Paper link:https://doi.org/10.1093/jxb/ery351

 

6. [2021 IF=11.277] An  Alternative  Splicing  Variant  of  PtRD26  Delays  Leaf  Senescence by Regulating Multiple NAC Transcription Factors in Populus.

Author: Hou-Ling Wang,Yi Zhang,Ting Wang, Qi Yang,Yanli Yang,Ze Li, Bosheng Li, Xing Wen, Wenyang Li, Weilun Yin, Xinli Xia, Hongwei Guo, and Zhonghai Li

Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

Journal: Plant Cell. Volume 33, Issue 5, May 2021, Pages 1594–1614

Institution:Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University

Paper link:https://doi.org/10.1093/plcell/koab046

 

7. [2021 IF=2.89] Rapid and sensitive detection ofStaphylococcus aureus and Klebsiella pneumonia based on bacitracin-modified Fe3O4@PDA magnetic beads combined with matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Author: Feng Qin, Shujuan Wang, Mingxia Gao  and  Xiangmin Zhang

Marker: RTD6110,彩虹预染超低分子量蛋白(1.2-45 kD)

Journal: Analytical Methods. 2021,13, 2804-2811

Institution:Department of Chemistry, Fudan University

Paper link:https://pubs.rsc.org/en/content/articlelanding/2021/ay/d1ay00614b

 

8. [2021 IF=3.74] Cloning, expression, and function of ferritins in the tick Haemaphysalis flava.

Author: Yu Zhao, Lei Liu, Jin-Bao Liu, Cong-Ying Wu, De-Yong Duan, Tian-Yin Cheng

Marker: RTD6142 高分子量非变性电泳蛋白质Marker II(45-669 kD)

Journal: Ticks and Tick-borne Diseases. Volume 13, Issue 2, March 2022, 101892

Institution:College of Veterinary Medicine, Hunan Agricultural University,

Paper link:https://linkinghub.elsevier.com/retrieve/pii/S1877959X21002454

 

9. [2021 IF=0.886] Purification and Characterization of an Intracellular β-Glucosidase from the Protoplast Fusant of Aspergillus oryzae and Aspergillus niger

Author: F.M. Zhu, B. Du, H.S. Gao, C.J. Liu, and J. Lia

Marker: RTD6109 高分子量蛋白质Marker(43-200 kD)

Journal: Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 6, pp. 626–632

Institution:College of Food Science and Technology, Hebei Normal University of Science and Technology

Paper link:https://link.springer.com/article/10.1134/S0003683810060116

 

10. [2021 IF=4.374] Effect of Fuzhuan brick-tea addition on the quality and antioxidant activity of skimmed set-type yoghurt.

Author: Dong Liu

Marker: RTD6103 低分子量蛋白质Marker II(14.4-116 kD)

Journal: International Journal of Dairy Technology, Vol 71 March 2018 Pages 22-33

Institution:Department of Food Processing Technology, Anqing Vocational & Technical College

Paper link:https://doi.org/10.1111/1471-0307.12395

 

11. [2021 IF=3.7] Smart delivery of poly-peptide composite for effective cancer therapy

Author: Xin Yuan, Yingzhou Qin, Qingmei Tian, Cuijuan Liu, Xiangzhou Meng, Bo Qie, Fan Gao, Ying Huang, Guanghui Xu, Guang Yang, Yimin Zhu

Marker: Protein Marker

Journal: Biomedical Materials, 2022 Jan 24;17(2)

Institution:School of Nano-Tech and Nano-Bionics, University of Science and Technology of China

Paper link:https://pubmed.ncbi.nlm.nih.gov/34996052/

 


 
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